stx 8 Search Results


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Full length Clone DNA of Human syntaxin 8 with C terminal GFPSpark tag.
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90
Proteintech syntaxin 8
Syntaxin 8, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stx+8/Syntaxin+8+Antibody/pmc06450627-33-46-49
Average 90 stars, based on 1 article reviews
syntaxin 8 - by Bioz Stars, 2026-10
90/100 stars
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93
Synaptic Systems rabbit serum anti stx8
Gp91 phox colocalizes with SNAREs on the phagosomal membrane. (A) Confocal images of dendritic cells stimulated with zymosan particles for 1 h and immunostained for gp91 phox (green in merge) together with Vti1b, stx7, VAMP8, <t>stx8,</t> SNAP23, stx12 or stx5 (magenta). Yellow arrowheads, phagosomes positive for both gp91 phox and the indicated SNARE. BF, brightfield. Scale bar: 5 µm. (B) Pearson correlation coefficients for colocalization of gp91 phox with the indicated SNAREs for zymosan-containing phagosomes (from panel A) and for unstimulated cells (w/o zymosan; Fig S2A ). Colocalization of gp91 phox immunostained with both Alexa-Fluor-488 and -568-labeled secondary antibodies is shown as a positive control (individual donors shown). (C) Significance levels (one-way ANOVA with Tukey's test) from panel B. Black text, zymosan-pulsed samples; red text, unstimulated samples. ns, not significant. (D) Normalized staining intensity values of phagosomal gp91 phox as a function of normalized intensity values of the indicated SNAREs for individual phagosomes (log2 scale; phagosomes pooled from >3 donors are shown; solid lines, linear regression with 95% confidence intervals; β, regression coefficients; R 2 , R-squared values). (E) Quantification of the phagosomal enrichment for gp91 phox and/or the indicated SNAREs (from panel D, with normalized intensity values >1). (F) Phagosomal enrichment of gp91 phox and the indicated SNAREs following stimulation with zymosan ( n =6 donors for gp91 phox ; n =3 donors for the SNAREs). Results show means±s.e.m.
Rabbit Serum Anti Stx8, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stx+8/110+083/pmc05399780-234-15-19
Average 93 stars, based on 1 article reviews
rabbit serum anti stx8 - by Bioz Stars, 2026-10
93/100 stars
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STX8 HEK293T cell transient overexpression lysate as WB positive control
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Stx8 GFP tagged Mouse syntaxin 8 cDNA clone MGC 58243 IMAGE 6774827
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Stx8 Myc DDK tagged Mouse syntaxin 8 Stx8
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STX8 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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STX8 KN2 0 Human gene knockout kit via CRISPR non homology mediated
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STX8 Antibody Biotin is a Rabbit Polyclonal against STX8 conjugated to Biotin
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Transient overexpression lysate of syntaxin 8 (STX8)
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Stx8 Rat 4 unique 29mer shRNA constructs in lentiviral GFP vector
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Image Search Results


Gp91 phox colocalizes with SNAREs on the phagosomal membrane. (A) Confocal images of dendritic cells stimulated with zymosan particles for 1 h and immunostained for gp91 phox (green in merge) together with Vti1b, stx7, VAMP8, stx8, SNAP23, stx12 or stx5 (magenta). Yellow arrowheads, phagosomes positive for both gp91 phox and the indicated SNARE. BF, brightfield. Scale bar: 5 µm. (B) Pearson correlation coefficients for colocalization of gp91 phox with the indicated SNAREs for zymosan-containing phagosomes (from panel A) and for unstimulated cells (w/o zymosan; Fig S2A ). Colocalization of gp91 phox immunostained with both Alexa-Fluor-488 and -568-labeled secondary antibodies is shown as a positive control (individual donors shown). (C) Significance levels (one-way ANOVA with Tukey's test) from panel B. Black text, zymosan-pulsed samples; red text, unstimulated samples. ns, not significant. (D) Normalized staining intensity values of phagosomal gp91 phox as a function of normalized intensity values of the indicated SNAREs for individual phagosomes (log2 scale; phagosomes pooled from >3 donors are shown; solid lines, linear regression with 95% confidence intervals; β, regression coefficients; R 2 , R-squared values). (E) Quantification of the phagosomal enrichment for gp91 phox and/or the indicated SNAREs (from panel D, with normalized intensity values >1). (F) Phagosomal enrichment of gp91 phox and the indicated SNAREs following stimulation with zymosan ( n =6 donors for gp91 phox ; n =3 donors for the SNAREs). Results show means±s.e.m.

Journal: Journal of Cell Science

Article Title: Oxidized phagosomal NOX2 complex is replenished from lysosomes

doi: 10.1242/jcs.196931

Figure Lengend Snippet: Gp91 phox colocalizes with SNAREs on the phagosomal membrane. (A) Confocal images of dendritic cells stimulated with zymosan particles for 1 h and immunostained for gp91 phox (green in merge) together with Vti1b, stx7, VAMP8, stx8, SNAP23, stx12 or stx5 (magenta). Yellow arrowheads, phagosomes positive for both gp91 phox and the indicated SNARE. BF, brightfield. Scale bar: 5 µm. (B) Pearson correlation coefficients for colocalization of gp91 phox with the indicated SNAREs for zymosan-containing phagosomes (from panel A) and for unstimulated cells (w/o zymosan; Fig S2A ). Colocalization of gp91 phox immunostained with both Alexa-Fluor-488 and -568-labeled secondary antibodies is shown as a positive control (individual donors shown). (C) Significance levels (one-way ANOVA with Tukey's test) from panel B. Black text, zymosan-pulsed samples; red text, unstimulated samples. ns, not significant. (D) Normalized staining intensity values of phagosomal gp91 phox as a function of normalized intensity values of the indicated SNAREs for individual phagosomes (log2 scale; phagosomes pooled from >3 donors are shown; solid lines, linear regression with 95% confidence intervals; β, regression coefficients; R 2 , R-squared values). (E) Quantification of the phagosomal enrichment for gp91 phox and/or the indicated SNAREs (from panel D, with normalized intensity values >1). (F) Phagosomal enrichment of gp91 phox and the indicated SNAREs following stimulation with zymosan ( n =6 donors for gp91 phox ; n =3 donors for the SNAREs). Results show means±s.e.m.

Article Snippet: Primary antibodies used were: mouse-IgG1 anti-gp91 phox (D162-3, MBL), rabbit serum anti-SNAP23 (111202, Synaptic Systems), rabbit serum anti-stx8 (110083, Synaptic Systems), rabbit serum anti-stx7 (110072, Synaptic Systems), mouse-IgG1 anti-stx7 (sc-514157, Santa Cruz), rabbit serum anti-stx12 (299022, Synaptic Systems), rabbit serum anti-VAMP8 (104302, Synaptic Systems), rabbit serum anti-Vti1b (164002, Synaptic Systems), rabbit serum anti-stx5 (110053, Synaptic Systems), rabbit serum anti-LAMP1 (L1418, Sigma), rabbit serum anti-TGN38 (sc-27680, Santa Cruz), mouse-IgG1 anti-EEA1 (610456, BD Biosciences), rabbit serum anti-p67phox (07-002, Merck Millipore), rabbit monoclonal-IgG anti-GAPDH (2118, Cell Signaling Technology), mouse-IgG1 anti-FITC (200-602-037, Jackson ImmunoResearch Laboratories) and mouse-IgG1 isotype control (400102, Biolegend) antibodies.

Techniques: Membrane, Labeling, Positive Control, Staining